It is well established that by reducing the ionic strength of a test system, there is an increase in the rate of red blood cell antigen-antibody binding.  The suspension of red cells in low ionic strength solution permits a substantial reduction in incubation time and also increases the test sensitivity with many antibody specificities.  The efficacy of low ionic strength suspending media in enhancing antigen-antibody reactions is dependent on their correct preparation and use.

The Biorex Diagnostics L.I.S.S. Ready for Use is a low ionic strength solution which is iso-osmotic with human red blood cells.  The solution contains glycine, phosphate buffer and 0.03M sodium chloride.